ion exchange chromatography membranes Search Results


sartobind lab q anion exchange membrane adsorbers  (Sartorius AG)
93
Sartorius AG sartobind lab q anion exchange membrane adsorbers
Sartobind Lab Q Anion Exchange Membrane Adsorbers, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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ion exchange chromatography membranes  (Vivascience Inc)
90
Vivascience Inc ion exchange chromatography membranes
Ion Exchange Chromatography Membranes, supplied by Vivascience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ion exchange chromatography membranes - by Bioz Stars, 2026-06
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mono q fast performance liquid chromatography fplc ion exchange chromatography solubilized plasma membrane  (Danaher Inc)
86
Danaher Inc mono q fast performance liquid chromatography fplc ion exchange chromatography solubilized plasma membrane
FIG. 4. Dephosphorylation of the insulin receptor by trans- membrane PTPases PTP-a and LAR isolated on a Mono Q col- umn. Adipocyte plasma membrane (2–3 mg) was solubilized in 1% Triton X-100 and then loaded onto a 5-cm Mono Q column. Fractions were eluted with a 0.05–1.0 M NaCl gradient using an Amersham Pharmacia Biotech <t>FPLC</t> apparatus. LAR and PTP-a were detected by Western blot with specific antibodies (A), and the arrow indicates the position of LAR (the rightmost lane is LAR from cells transfected as in Ref. 21). Column fractions were pooled, concentrated, and assayed for a PTPase activity with autophosphorylated, 32P-labeled insulin receptor (10 mg of total protein, ;0.1–0.5 mg of receptor) as a substrate (B). The results are representative of two separate experiments.
Mono Q Fast Performance Liquid Chromatography Fplc Ion Exchange Chromatography Solubilized Plasma Membrane, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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FIG. 4. Dephosphorylation of the insulin receptor by trans- membrane PTPases PTP-a and LAR isolated on a Mono Q col- umn. Adipocyte plasma membrane (2–3 mg) was solubilized in 1% Triton X-100 and then loaded onto a 5-cm Mono Q column. Fractions were eluted with a 0.05–1.0 M NaCl gradient using an Amersham Pharmacia Biotech FPLC apparatus. LAR and PTP-a were detected by Western blot with specific antibodies (A), and the arrow indicates the position of LAR (the rightmost lane is LAR from cells transfected as in Ref. 21). Column fractions were pooled, concentrated, and assayed for a PTPase activity with autophosphorylated, 32P-labeled insulin receptor (10 mg of total protein, ;0.1–0.5 mg of receptor) as a substrate (B). The results are representative of two separate experiments.

Journal: The Journal of biological chemistry

Article Title: Dynamics of protein-tyrosine phosphatases in rat adipocytes.

doi: 10.1074/jbc.275.9.6308

Figure Lengend Snippet: FIG. 4. Dephosphorylation of the insulin receptor by trans- membrane PTPases PTP-a and LAR isolated on a Mono Q col- umn. Adipocyte plasma membrane (2–3 mg) was solubilized in 1% Triton X-100 and then loaded onto a 5-cm Mono Q column. Fractions were eluted with a 0.05–1.0 M NaCl gradient using an Amersham Pharmacia Biotech FPLC apparatus. LAR and PTP-a were detected by Western blot with specific antibodies (A), and the arrow indicates the position of LAR (the rightmost lane is LAR from cells transfected as in Ref. 21). Column fractions were pooled, concentrated, and assayed for a PTPase activity with autophosphorylated, 32P-labeled insulin receptor (10 mg of total protein, ;0.1–0.5 mg of receptor) as a substrate (B). The results are representative of two separate experiments.

Article Snippet: Purification of PTPases by a Mono-Q Fast Performance Liquid Chromatography (FPLC) Ion Exchange Chromatography—Solubilized plasma membrane (2–3 mg of protein) was subjected to FPLC with a Mono Q column (HR5/5; Amersham Pharmacia Biotech).

Techniques: De-Phosphorylation Assay, Membrane, Isolation, Clinical Proteomics, Western Blot, Transfection, Activity Assay, Labeling